Simultaneous detection and differentiation of respiratory syncytial virus and other respiratory viral pathogens.
Identifieur interne : 002040 ( Main/Exploration ); précédent : 002039; suivant : 002041Simultaneous detection and differentiation of respiratory syncytial virus and other respiratory viral pathogens.
Auteurs : Susan E. Sefers [États-Unis] ; Haijing Li ; Yi-Wei TangSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2011.
Descripteurs français
- KwdFr :
- Appareil respiratoire (virologie), Humains, Infections de l'appareil respiratoire (diagnostic), Infections de l'appareil respiratoire (virologie), Réaction de polymérisation en chaîne (), Séquençage par oligonucléotides en batterie (), Tests de criblage à haut débit (), Tests de criblage à haut débit (instrumentation), Virus respiratoires syncytiaux (isolement et purification).
- MESH :
- diagnostic : Infections de l'appareil respiratoire.
- isolement et purification : Virus respiratoires syncytiaux.
- virologie : Appareil respiratoire, Infections de l'appareil respiratoire.
- instrumentation : Humains, Réaction de polymérisation en chaîne, Séquençage par oligonucléotides en batterie, Tests de criblage à haut débit.
English descriptors
- KwdEn :
- High-Throughput Screening Assays (instrumentation), High-Throughput Screening Assays (methods), Humans, Oligonucleotide Array Sequence Analysis (methods), Polymerase Chain Reaction (methods), Respiratory Syncytial Viruses (isolation & purification), Respiratory System (virology), Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (virology).
- MESH :
- diagnosis : Respiratory Tract Infections.
- instrumentation : High-Throughput Screening Assays.
- isolation & purification : Respiratory Syncytial Viruses.
- methods : High-Throughput Screening Assays, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction.
- virology : Respiratory System, Respiratory Tract Infections.
- Humans.
Abstract
Rapid and accurate detection of respiratory syncytial virus (RSV) provides pathogen-specific diagnosis, allows implementation of appropriate infection control measures, and improves patient management. One diagnostic challenge is that respiratory infections, which can be caused by several viral pathogens including RSV, usually present with similar signs and symptoms that are nearly indistinguishable by clinical diagnosis. We have described in the chapter a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens in one single reaction. With the combination of target-enriched multiplexing PCR amplification and Luminex suspension array identification, 12 common respiratory viruses, including RSV A and B, influenza virus A and B, parainfluenza virus 1, 2, 3, and 4, human metapneumovirus, rhinoviruses, enteroviruses, and SARS coronavirus, are detected and differentiated simultaneously within five hours.
DOI: 10.1007/978-1-60761-817-1_17
PubMed: 21116808
Affiliations:
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Le document en format XML
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One diagnostic challenge is that respiratory infections, which can be caused by several viral pathogens including RSV, usually present with similar signs and symptoms that are nearly indistinguishable by clinical diagnosis. We have described in the chapter a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens in one single reaction. With the combination of target-enriched multiplexing PCR amplification and Luminex suspension array identification, 12 common respiratory viruses, including RSV A and B, influenza virus A and B, parainfluenza virus 1, 2, 3, and 4, human metapneumovirus, rhinoviruses, enteroviruses, and SARS coronavirus, are detected and differentiated simultaneously within five hours.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><noCountry><name sortKey="Li, Haijing" sort="Li, Haijing" uniqKey="Li H" first="Haijing" last="Li">Haijing Li</name><name sortKey="Tang, Yi Wei" sort="Tang, Yi Wei" uniqKey="Tang Y" first="Yi-Wei" last="Tang">Yi-Wei Tang</name></noCountry><country name="États-Unis"><region name="Tennessee"><name sortKey="Sefers, Susan E" sort="Sefers, Susan E" uniqKey="Sefers S" first="Susan E" last="Sefers">Susan E. 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